Overview
Vilon 20 mg (KE; Lys–Glu) from Peptide Tech is a lyophilized, research-use-only (RUO) dipeptide supplied at ≥99% purity (HPLC & MS verified). This minimal L-lysyl-L-glutamyl sequence is packaged for precise in-vitro work in chromatin biology, cytokine gene-expression models, and peptide analytics where documentation, batch traceability, and cold-chain integrity matter.
Specifications
| Property | Details |
|---|---|
| Product Type | Synthetic dipeptide (lyophilized powder) |
| Chemical Name | N-L-Lysyl-L-glutamic acid |
| Synonyms | Vilon; Lysylglutamic acid; Lys-Glu; H-Lys-Glu-OH; “KE” |
| Sequence | Lys-Glu (K-E) |
| CAS Number | 45234-02-4 |
| PubChem CID | 7010502 |
| Molecular Formula | C11H21N3O5 |
| Molecular Weight | ≈ 275.30 g/mol |
| Appearance | White to off-white lyophilized powder |
| Content per Vial | 20 mg |
| Container | 3 mL Type I glass vial |
| Purity / Identity | ≥99% by HPLC; identity by MS (batch COA via on-vial QR) |
| Solubility | Freely soluble in sterile water or neutral aqueous buffers; a brief pre-wet with ≤0.1% acetic acid can aid rapid dissolution. If your SOP requires sterility, 0.22 µm filter after reconstitution. |
| Intended Use | Laboratory research only (RUO). Not for human or animal use. |
Molecular Structure
Key Features of Peptide Tech
- Same-Day Shipping (Mon–Fri, on in-stock items)
- GMP-Aligned lyophilization & documentation
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- 24/7 Support
- Price Match Guarantee
- Batch Tracking with on-vial QR verification
- 3rd-Party U.S. Lab Testing — purity, quantity, endotoxin
- Cold-Chain Shipping — Always (no paid upgrade)
- Worldwide Shipping
- All Major Payments Accepted
- Text & Email Order Updates
- 2-Day FedEx/UPS Standard with low-cost overnight
- Largest Variety of peptides & bioregulators
Potential Applications in Research
Strictly for in-vitro, cell-based, or biochemical experiments. No health, diagnostic, or therapeutic claims.
- Chromatin state assays in lymphocytes — evaluate facultative/structural heterochromatin dynamics (e.g., DAPI densitometry, NOR silver staining, immunocytochemistry) in cultured human lymphocytes under defined conditions. :contentReference[oaicite:0]{index=0}
- IL-2 gene-expression models — quantify peptide-driven changes in Il2 mRNA in mouse splenocytes (qPCR), including time-/dose-responses and promoter-reporter readouts. :contentReference[oaicite:1]{index=1}
- Pathway mapping around IL-2 signaling — pair IL-2 transcription readouts with downstream pathway markers (e.g., STAT5 phosphorylation) to contextualize responses in controlled media. (Background review support.) :contentReference[oaicite:2]{index=2}
- Peptide-transporter (PepT1) uptake screens — use heterologous systems or cell lines expressing SLC15A1 to profile charged dipeptide handling and structure–activity determinants. (General transporter methodology.) :contentReference[oaicite:3]{index=3}
- Analytical method development — RP-HPLC/LC–MS/MS workflows for identity, stability, recovery, and matrix effects across buffers and temperatures; establish assay-ready reference material and retention/fragmentation anchors (per COA).
Citations (MLA)
- Lezhava, Teimuraz, et al. “Bioregulator Vilon-Induced Reactivation of Chromatin in Cultured Lymphocytes from Old People.” Biogerontology, vol. 5, no. 2, 2004, pp. 73–79. https://doi.org/10.1023/B:BGEN.0000025070.90330.7f. :contentReference[oaicite:4]{index=4}
- Khavinson, V. Kh., et al. “Effect of Peptide Lys-Glu on Interleukin-2 Gene Expression in Lymphocytes.” Bulletin of Experimental Biology and Medicine, vol. 130, no. 3, 2000, pp. 898–899. https://doi.org/10.1007/BF02682264. :contentReference[oaicite:5]{index=5}
- Korneva, E. A., et al. “In Vitro Effect of Short Peptides on Expression of Interleukin-2 Gene in Splenocytes of CBA Mice.” Bulletin of Experimental Biology and Medicine, vol. 133, 2002, pp. 186–188. https://doi.org/10.1023/A:1020210615148. :contentReference[oaicite:6]{index=6}
- Boyman, Onur, and Jonathan Sprent. “The Role of Interleukin-2 in Immunity and Tolerance.” Trends in Immunology, vol. 33, no. 7, 2012, pp. 371–377. Link. (Background for IL-2 pathway assays.) :contentReference[oaicite:7]{index=7}
- Verri, T., et al. “The Teleost PepT1-Type Peptide Transporters and Their Specificity.” Frontiers in Physiology, 2023. Link. (General transporter methodology context.) :contentReference[oaicite:8]{index=8}
Quality, Storage & Handling
Every lot is confirmed by HPLC (purity) and MS (identity); the on-vial QR links to the batch COA. Independent U.S. labs verify purity, quantity, and endotoxin.
- Storage (lyophilized): −20 °C long-term or 2–8 °C short-term; keep desiccated and protected from light.
- After reconstitution: Aliquot and store at ≤ −20 °C (or −80 °C per SOP); avoid repeated freeze–thaw cycles.
- Reconstitution tips: Use sterile water or compatible buffer; gently swirl/invert to dissolve. If sterility is required, pass through a 0.22 µm filter and aliquot promptly.
Shipping & Fulfillment
Ships with validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.
Compliance & RUO Disclaimer
For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines.
FAQs
- Is Vilon the same as Thymogen?
- No. Vilon is the dipeptide Lys-Glu (KE), whereas Thymogen is Glu-Trp (EW). Verify the exact sequence required by your assay.
- What purity do you provide?
- Each 20 mg vial is ≥99% by HPLC with MS identity confirmation; third-party testing is performed on every lot (COA via on-vial QR).
- How should I reconstitute it?
- Dissolve in sterile water or compatible buffer; gently invert until fully dissolved. If your SOP requires sterility, 0.22 µm filter and aliquot to avoid repeat freeze–thaw.
- Which in-vitro assays are common?
- Typical RUO uses include chromatin-state readouts in lymphocytes, IL-2 mRNA reporter/qPCR experiments, and general HPLC/LC–MS method development, with optional PepT1 uptake panels for charged dipeptides.
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Always read the label and use products as directed. Consult a qualified professional where appropriate.













