NAD+ 500mg

Specifications

Molecular Structure

Key Features of Peptide Tech

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• 3rd-Party U.S. Lab Testing — purity, quantity, endotoxin
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Potential Applications in Research

Strictly in-vitro, cell-free, or cell-based workflows. No health claims or clinical/veterinary use.

• Oxidoreductase enzyme kinetics (e.g., LDH, MDH, ADH) monitoring NADH formation at 340 nm or via fluorescence to determine V_max/K_m and inhibitor effects.
• Sirtuin deacetylase assays that consume NAD⁺ to generate OAADPr and nicotinamide, quantified by LC–MS or homogeneous fluorescence kits.
• PARP/ARTD ADP-ribosylation workflows (auto- and trans-modification) with immuno or LC–MS readouts to map poly/mono-(ADP-ribose) formation.
• NAD⁺-dependent DNA ligase assays (LigA) for nick-sealing efficiency, cofactor dependence, and inhibitor screening.
• Metabolomics method development (LC–MS/MS) for redox-pair profiling of NAD⁺/NADH and NADP(H) in buffered matrices.
• Assay calibration & stability studies (RP-HPLC/LC–MS) establishing retention, ionization, and recovery of dinucleotides.

Quality, Storage & Handling

Each lot is confirmed by HPLC (purity) and MS (identity); COA and batch data are accessible via QR. Independent U.S. labs verify purity, quantity, and endotoxin.

• Storage (lyophilized): −20 °C long-term or 2–8 °C short-term; keep desiccated and protected from light.
• After reconstitution: Use neutral, oxygen-poor buffer when possible; aliquot and store at ≤−20 °C; avoid repeated freeze–thaw.
• Working solutions: Prepare fresh; minimize exposure to light and high pH to reduce degradation.

Shipping & Fulfillment

Ships with validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Disclaimer

For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines.

FAQs

Is this the oxidized form?

Yes. This product is NAD⁺ (oxidized). For reduced NADH or NADP(H), see our catalog.

What purity is provided?

Each 500 mg vial is ≥99% by HPLC with MS identity confirmation; third-party testing is performed on every lot (COA via QR).

How should I reconstitute it?

Use sterile water or a neutral, compatible buffer; gently invert to dissolve. Prepare fresh working solutions, aliquot, and avoid alkaline pH and repeated freeze–thaw.

Which in-vitro assays is NAD⁺ commonly used in?

Typical RUO uses include dehydrogenase kinetics (340 nm), sirtuin and PARP assays, NAD⁺-dependent ligase reactions, and LC–MS method development for dinucleotides.

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