PNC-27 10mg

PNC-27 10 mg from Peptide Tech is a chimeric, lyophilized research-use-only (RUO) peptide that fuses the p53 (residues 12–26) HDM2-binding motif to the cell-penetrating peptide penetratin. Each lot is supplied at ≥99% purity (HPLC & MS verified) with batch traceability for reproducible in-vitro mechanistic studies (membrane interaction, target engagement, pore-formation models) and peptide analytics.

 

Specifications

Property	Details
Product	PNC-27 — lyophilized powder (10 mg)
Chemical Class	Chimeric peptide: p5312–26 motif + penetratin leader
CAS Number	1159861-00-3
PubChem	No consolidated entry for the full chimera
Sequences	p53 (aa 12–26): PPLSQETFSDLWKLL Penetratin: RQIKIWFQNRRMKWKK Chimera: PPLSQETFSDLWKLL–RQIKIWFQNRRMKWKK
Appearance	White to off-white lyophilized powder
Container	3 mL Type I glass vial, butyl stopper, flip-off seal
Purity / Identity	≥99% by HPLC; identity by MS (batch COA via on-vial QR)
Counter-ion	Typically acetate; verify on lot COA
Solubility	Soluble in sterile water; for concentrated stocks, pre-wet with ≤0.1% acetic acid then bring to buffer (e.g., PBS). If required by SOP, 0.22 µm filter after reconstitution.
Storage (lyophilized)	−20 °C long-term (desiccated, light-protected)
After Reconstitution	Aliquot; store at ≤−20 °C (or −80 °C per SOP); avoid repeat freeze–thaw
Intended Use	RUO only. Not for human or animal use.

Molecular Structure

There is no single PubChem 2D rendering for the complete PNC-27 chimera. Component sequences are provided above; lot-specific analytical data (HPLC/MS) and identifiers are available on the QR-linked COA.

Key Features of Peptide Tech

• Same-Day Shipping (Mon–Fri, on in-stock items)
• GMP-Aligned lyophilization & documentation
• USA Made
• 24/7 Support
• Price Match Guarantee
• Batch Tracking with QR Code Verification
• 3rd-Party U.S. Lab Testing — purity, quantity, endotoxin
• Cold-Chain Shipping — Always (no paid upgrade)
• Worldwide Shipping
• All Major Payments Accepted
• Text & Email Order Updates
• 2-Day FedEx/UPS Standard with low-cost overnight
• Largest Variety of peptides & bioregulators

Potential Applications in Research

Strictly for in-vitro, cell-based, or biochemical experiments. No health, diagnostic, or therapeutic claims.

• HDM2 interaction & localization — co-IP, proximity assays, and membrane colocalization in lines reported to present membrane-associated HDM2.
• Membrane pore-formation models — liposome leakage, GUV disruption, bilayer conductance to dissect peptide-induced membranolysis.
• Cellular cytotoxicity profiling — LDH release, caspase readouts, and live-cell imaging to compare necrosis vs. apoptosis under controlled conditions.
• Structure–function mapping — compare full chimera vs. segments (p53 motif alone, penetratin alone) and scrambled controls.
• Target-dependency studies — correlate HDM2 presence/compartmentalization with in-vitro sensitivity.

Citations (MLA)

1. Sarafraz-Yazdi, E., et al. “Anticancer Peptide PNC-27 Adopts an HDM-2-Binding Conformation and Kills Cancer Cells by Binding to HDM-2 in Their Membranes.” PNAS, 107(5), 2010, 1918–1923. https://doi.org/10.1073/pnas.0909364107
2. Pincus, M. R., et al. “The Anti-Cancer Peptide, PNC-27, Induces Tumor Cell Lysis as the Intact Peptide.” Cancer Chemotherapy and Pharmacology, 66(2), 2010, 325–331. https://doi.org/10.1007/s00280-009-1166-7
3. Davitt, J. G., et al. “The Anti-Cancer Peptide, PNC-27, Induces Tumor Cell Necrosis of a Poorly Differentiated Non-Solid Tissue Human Leukemia Cell Line …” Annals of Clinical & Laboratory Science, 44(3), 2014, 241–248.
4. Sarafraz-Yazdi, E., et al. “PNC-27, a Chimeric p53-Penetratin Peptide Binds to HDM-2 in a p53 Peptide-Like Structure …” Biomedicines, 10(5), 2022, 945. https://doi.org/10.3390/biomedicines10050945

Quality, Handling & Experimental Notes

• Verification: Independent U.S. labs validate purity, identity, quantity, and endotoxin; COA linked via on-vial QR.
• Reconstitution: Bring vial to RT; add sterile water (or ≤0.1% acetic acid for rapid wetting) then adjust into assay buffer. Avoid vigorous vortexing; use gentle swirl or brief sonication.
• Aliquoting: Prepare single-use aliquots to minimize freeze–thaw; store frozen.
• Controls: Include scrambled or segment-only controls (p53 motif alone, penetratin alone) for mechanistic interpretation.

Shipping & Fulfillment

Ships with validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Notice

For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Not FDA-approved. Purchaser warrants appropriate qualifications and compliance with applicable regulations and institutional policies.

FAQs

Is this suitable for in-vivo use?

No. This product is supplied strictly as RUO and must not be administered to humans or animals.

How do I verify my vial?

Scan the on-vial QR code to access the batch COA with HPLC/MS data and third-party analytics.

Which buffer should I use?

Most groups start in sterile water, then adjust into isotonic buffers (e.g., PBS) at the target pH. Confirm compatibility with your assay and include appropriate controls.

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