PE-22-28 10mg

PE-22-28 10 mg (Mini-Spadin; H-Gly-Val-Ser-Trp-Gly-Leu-Arg-OH) from Peptide Tech is a premium, lyophilized research-use-only (RUO) heptapeptide supplied at ≥99% purity (HPLC & MS verified). This spadin-derived fragment (GVSWGLR) is formatted for reproducible in-vitro K₂P channel pharmacology, electrophysiology, and method-development workflows where sequence fidelity, batch traceability, and cold-chain integrity are critical.

 

Specifications

Property	Details
Product	PE-22-28 — lyophilized peptide, 10 mg
Chemical Name	H-Gly-Val-Ser-Trp-Gly-Leu-Arg-OH (free acid)
Synonyms	Mini-Spadin; Spadin analog; GVSWGLR; PE 22-28
Sequence	Gly–Val–Ser–Trp–Gly–Leu–Arg (GVSWGLR)
CAS Number	1801959-12-5
PubChem CID	165437303
Molecular Formula	C35H55N11O9
Molecular Weight	≈ 773.9 g/mol
Appearance	White to off-white lyophilized powder
Container	3 mL Type I glass vial; butyl stopper; flip-off seal
Purity / Identity	≥99% by HPLC; identity by MS (batch COA via on-vial QR)
Counter-ion	Typically acetate (verify on lot COA)
Solubility	Soluble in sterile water or neutral buffers; for rapid wetting at high concentration, pre-wet with ≤0.1% acetic acid and bring to buffer. If sterility is required by your SOP, filter through 0.22 µm.
Storage (lyophilized)	−20 °C long-term; store desiccated and protected from light
After Reconstitution	Aliquot and store at ≤ −20 °C (or −80 °C per SOP); avoid repeat freeze–thaw cycles
Intended Use	RUO only. Not for human or animal use

Molecular Structure

Key Features of Peptide Tech

• Same-Day Shipping (Mon–Fri, on in-stock items)
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• 3rd-Party U.S. Lab Testing — purity, quantity, endotoxin
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Potential Applications in Research (RUO)

Strictly for laboratory research. No health, diagnostic, or therapeutic claims.

• TREK-1 (K₂P2.1) channel assays — whole-cell patch-clamp on HEK293 cells stably expressing human TREK-1 to quantify inhibition curves (IC₅₀) vs. arachidonic-acid-activated currents and to compare analogs of the 7-mer core. :contentReference[oaicite:0]{index=0}
• Selectivity profiling across K₂P — evaluate activity at TREK-2, TRAAK, TRESK, and TASK-1 in matched heterologous systems to confirm specificity reported for spadin analogs. :contentReference[oaicite:1]{index=1}
• Structure–activity exploration — test N-/C-terminal modifications or residue substitutions of GVSWGLR for potency and stability changes in vitro; develop SAR libraries around the PE-22-28 core. :contentReference[oaicite:2]{index=2}
• Neuronal excitability readouts — in cultured neuronal models, pair channel modulation with downstream markers (e.g., membrane potential imaging) under tightly controlled, in-vitro conditions. :contentReference[oaicite:3]{index=3}
• Analytical method development — implement stability-indicating RP-HPLC/LC–MS(/MS) for identity, purity, and forced-degradation studies; quantify recovery from buffers and media. (General methods; see peer-reviewed electrophysiology for assay framing.) :contentReference[oaicite:4]{index=4}

Citations (selected)

1. Djillani, Alaeddine, et al. “Shortened spadin analogs display better TREK-1 inhibition, in vivo stability and antidepressant activity.” Frontiers in Pharmacology, vol. 8, 2017, article 643. https://doi.org/10.3389/fphar.2017.00643.
2. Mazella, Jean, et al. “Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design.” PLOS Biology, vol. 8, no. 4, 2010, e1000355. https://doi.org/10.1371/journal.pbio.1000355.
3. Pietri, Mariel, et al. “First evidence of protective effects on stroke recovery and post-stroke depression induced by sortilin-derived peptides.” Neuropharmacology, vol. 158, 2019, 107715. https://doi.org/10.1016/j.neuropharm.2019.107715.

Quality, Storage & Handling

Each lot is confirmed by HPLC (purity) and MS (identity); the on-vial QR links to the batch COA. Independent U.S. labs verify purity, quantity, and endotoxin.

• Storage (lyophilized): −20 °C long-term or 2–8 °C short-term; keep desiccated and protected from light.
• After reconstitution: Aliquot promptly; store at ≤ −20 °C (or −80 °C per SOP). Avoid multiple freeze–thaw cycles.
• Reconstitution tips: Use sterile water or suitable buffer; gently swirl/invert to dissolve. If sterility is required, 0.22 µm filter and aliquot.

Shipping & Fulfillment

Ships with validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Disclaimer

For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Purchasers warrant appropriate qualifications and compliance with applicable regulations and institutional policies.

FAQs

Is PE-22-28 the same as spadin?

No. Spadin is a longer sortilin-derived peptide; PE-22-28 is a shortened 7-mer fragment (GVSWGLR) identified as a potent TREK-1 blocker for in-vitro studies. :contentReference[oaicite:8]{index=8}

Do you provide a PubChem image?

Yes—the 2D structure above is rendered from PubChem CID 165437303 for PE-22-28.

What purity do you provide?

Each 10 mg vial is ≥99% by HPLC with MS identity confirmation; third-party testing is performed on every lot (COA via on-vial QR).

How should I reconstitute it?

Dissolve in sterile water or a compatible buffer; gently invert until fully dissolved. If your SOP requires sterility, 0.22 µm filter and aliquot to avoid repeat freeze–thaw.

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