Overview
Specifications
| Property | Details |
|---|---|
| CAS Number | 89030-95-5 (GHK-Cu complex) |
| Chemical Name | Glycyl-L-histidyl-L-lysine copper(II) (copper tripeptide) |
| PubChem CID | GHK-Cu (CID 378611); Prezatide copper acetate (CID 71587328) |
| Molecular Formula | C14H24CuN6O4 (complex) |
| Molecular Weight | ≈ 403.9 g/mol (complex, no counter-ion) |
| Physical Appearance | White to faint blue lyophilized powder |
| Sequence | Gly-His-Lys (tri-peptide, Cu(II) chelate) |
| Container | 3 mL glass vial (50 mg net content) |
| Purity | ≥99% verified by HPLC & MS |
| Solubility | Readily soluble in sterile water or aqueous buffers; complex speciation is pH-dependent—avoid strong chelators (e.g., EDTA) in assay buffers |
| Intended Use | Laboratory research only (RUO). Not for human or animal use. |
Molecular Structure
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Potential Applications in Research
Strictly in-vitro, cellular, or biochemical contexts; no clinical or animal claims.
- ECM marker assays in dermal fibroblasts (e.g., collagen I/III, elastin, and GAG readouts via colorimetric or immunoassay methods).
- Cell migration and scratch-closure models in keratinocytes/fibroblasts to profile peptide–metal effects on wound-gap kinetics under serum-controlled conditions.
- MMP/TIMP balance studies using gelatin/collagen zymography or ELISA to quantify matrix remodeling signatures.
- Peptide–metal chelation and redox assays (UV-Vis/fluorimetry) to characterize Cu(II) binding stoichiometry, stability constants, and antioxidant surrogate activity in buffer systems.
- Copper uptake/handling workflows in cell culture (non-chelating buffers) to track intracellular copper proxies and downstream transcriptional responses.
Quality, Storage & Handling
Each lot is confirmed by HPLC (purity) and MS (identity); QR on the vial links to the batch COA. Independent U.S. labs verify purity, quantity, and endotoxin.
- Storage (lyophilized): −20 °C long-term or 2–8 °C short-term; keep desiccated and protected from light.
- After reconstitution: Aliquot and store at ≤−20 °C (or −80 °C per SOP); avoid repeated freeze–thaw.
- Buffer notes: Use non-chelating buffers; avoid EDTA and strong competing ligands that can disrupt the Cu(II) complex.
Shipping & Fulfillment
Ships in validated cold-chain packaging. In-stock orders placed before the daily cutoff dispatch the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.
Compliance & RUO Disclaimer
For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines. COAs available via QR or upon request.
FAQs
- Is this intended for human or veterinary use?
- No. This product is sold strictly as RUO and must not be used in humans or animals.
- What purity do you provide?
- Each 50 mg vial is ≥99% by HPLC with MS identity confirmation; third-party testing is performed on every lot (see COA via QR).
- How should I reconstitute it?
- Use sterile water or a non-chelating buffer; gently invert until fully dissolved. If required, 0.22 µm filter-sterilize and aliquot to avoid repeat freeze–thaw.
- Which in-vitro assays is GHK-Cu commonly used in?
- Typical RUO applications include ECM marker assays (collagen/elastin), scratch-closure models, MMP/TIMP profiling, and chelation/redox characterizations.
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