Thymalin 20 mg (EW; Glu–Trp) from Peptide Tech is a premium, lyophilized research‑use‑only (RUO) dipeptide supplied at ≥99% purity (HPLC & MS verified). The sequence H‑Glu‑Trp‑OH is prepared for reproducible in‑vitro immunology/epithelium models and analytical method development—backed by batch traceability, independent third‑party testing, and validated cold‑chain logistics.
Specifications
| Property | Details |
|---|---|
| Product | Thymalin — lyophilized peptide, 20 mg |
| Chemical Name | H‑Glu‑Trp‑OH (L‑glutamyl‑L‑tryptophan) |
| Synonyms | EW; Glu‑Trp; Thymogen‑like dipeptide |
| Sequence | Glu–Trp (E‑W) |
| CAS Number | 38101‑59‑6 (free acid) |
| PubChem CID | 3081371 (L‑Glu‑L‑Trp) |
| Molecular Formula | C16H19N3O5 (free acid) |
| Molecular Weight | ≈ 333.34 g/mol (free acid) |
| Appearance | White to off‑white lyophilized powder |
| Container | 3 mL Type I glass vial; butyl stopper; flip‑off seal |
| Purity / Identity | ≥99% by HPLC; identity by MS (batch COA via on‑vial QR) |
| Counter‑ion | Typically acetate (verify on lot COA) |
| Solubility | Soluble in sterile water or neutral buffers; for rapid wetting at higher concentrations, pre‑wet with ≤0.1% acetic acid then bring to buffer. If sterility is required by your SOP, 0.22 µm filter after dissolution. |
| Storage (lyophilized) | −20 °C long‑term; store desiccated and protected from light. |
| After Reconstitution | Aliquot and store at ≤ −20 °C (or −80 °C per SOP); avoid repeat freeze–thaw cycles. |
| Intended Use | RUO only. Not for human or animal use. |
Molecular Structure
Key Features of Peptide Tech
- Same‑Day Shipping (Mon–Fri, on in‑stock items)
- GMP‑Aligned lyophilization & documentation
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- Batch QR for instant COA & chain‑of‑custody verification
- 3rd‑Party U.S. Lab Testing — purity, quantity, endotoxin
- Cold‑Chain Shipping — Always (no paid upgrade)
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- Text & Email order updates; 2‑Day FedEx/UPS standard with low‑cost overnight
- Largest Variety of peptides & bioregulators
Potential Applications in Research (RUO)
Strictly for laboratory research. No health, diagnostic, or therapeutic claims.
- Immune‑cell signaling — quantify cytokine panels (e.g., IL‑6, TNF‑α, IL‑10) in macrophage or T‑cell cultures after defined EW exposures in serum‑controlled media.
- Epithelial barrier & wound closure — TEER and scratch‑assay paradigms in airway/intestinal lines to evaluate barrier dynamics under standardized conditions.
- Oxidative‑stress workflows — ROS quantitation, antioxidant transcripts, and mitochondrial potential in epithelial or immune models across media compositions.
- Peptide transport & stability — characterize uptake and stability of short acidic dipeptides in Caco‑2/HEK systems; quantify by LC–MS(/MS).
- Analytical method development — establish RP‑HPLC/LC–MS(/MS) identity, purity, recovery, and forced‑degradation stability across buffers, pH, and temperature.
Citations (selected)
- Khavinson, V. Kh., et al. Short peptide bioregulators — background on thymus‑related dipeptides and epithelial/immune applications (review context).
- Brandsch, M. PepT1/2 peptide transporters — methodology for di‑/tripeptide uptake studies.
- Standard TEER/scratch, ROS, and LC–MS quantitation protocols (Methods in Molecular Biology/JOVE style references).
Quality, Storage & Handling
Every lot is confirmed by HPLC (purity) and MS (identity); the on‑vial QR links to the batch COA. Independent U.S. labs verify purity, quantity, and endotoxin.
- Storage (lyophilized): −20 °C long‑term or 2–8 °C short‑term; keep desiccated and protected from light.
- After reconstitution: Aliquot promptly; store at ≤ −20 °C (or −80 °C per SOP). Avoid multiple freeze–thaw cycles.
- Reconstitution tips: Use sterile water or suitable buffer; gently swirl/invert to dissolve. If sterility is required, 0.22 µm filter and aliquot.
Shipping & Fulfillment
Ships with validated cold‑chain packaging. In‑stock orders placed before the daily cutoff ship the same day. 2‑Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.
Compliance & RUO Disclaimer
For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Purchasers warrant appropriate qualifications and compliance with applicable regulations and institutional policies.
FAQs
- Is Thymalin the same as Vilon or Thymogen?
- No. Thymalin is presented here as the dipeptide Glu–Trp (EW). Vilon is Lys–Glu (KE) and Thymogen refers to Glu–Trp in some literature but is often a branded preparation; verify the exact sequence required by your assay.
- Do you provide a PubChem image?
- Yes—the 2D structure above is rendered from PubChem CID 3081371 for L‑Glu‑L‑Trp (EW).
- What purity do you provide?
- Each 20 mg vial is ≥99% by HPLC with MS identity confirmation; third‑party testing is performed on every lot (COA via on‑vial QR).
- How should I reconstitute it?
- Dissolve in sterile water or a compatible buffer; gently invert until fully dissolved. If your SOP requires sterility, 0.22 µm filter and aliquot to avoid repeat freeze–thaw.
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