Cardiogen (AEDR) 20 mg from Peptide Tech is a premium, lyophilized research-use-only (RUO) tetrapeptide supplied at ≥99% purity (HPLC & MS verified). The sequence H-Ala-Glu-Asp-Arg-OH (1-letter: A-E-D-R) is widely explored for in-vitro studies involving fibroblast biology, cardiomyocyte cell models, and peptide analytics—where sequence fidelity, documentation, and cold-chain integrity are essential.

Specifications

Property	Details
Product	Cardiogen (AEDR) — 20 mg
Chemical Name	H-Ala-Glu-Asp-Arg-OH (free acid)
Synonyms	AEDR tetrapeptide; “Cardiogen”
CAS Number	Not assigned / varies by registrant (verify per lot COA)
PubChem CID	11583989
Molecular Formula	C₁₈H₃₁N₇O₉
Molecular Weight	≈ 489.5 g/mol
Sequence	Ala–Glu–Asp–Arg (A-E-D-R)
Physical Appearance	White to off-white lyophilized powder
Container	3 mL Type I glass vial (20 mg net content)
Purity / ID	≥99% by HPLC; identity by MS (batch COA via on-vial QR)
Solubility	Soluble in sterile water or neutral aqueous buffers; prepare fresh. If required by your SOP, 0.22 µm filter after reconstitution.
Intended Use	Laboratory research only (RUO). Not for human or animal use.

Molecular Structure

Key Features of Peptide Tech

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Potential Applications in Research

Strictly for in-vitro, cell-based, or biochemical experiments. No health, diagnostic, or therapeutic claims.

Fibroblast signaling & cytoskeletal protein expression — quantify actin, tubulin, vimentin, and lamin A/C responses to AEDR in cultured fibroblasts (e.g., immunoblot/IF), building on reports of tetrapeptide-driven protein synthesis changes. :contentReference[oaicite:0]{index=0}
Cardiomyocyte stress-response panels — assess viability, caspase activity, and apoptosis-related markers (e.g., p53 axis) under controlled stressors (hypoxia/reoxygenation, oxidative challenge) in neonatal/hiPSC-derived cardiomyocytes. :contentReference[oaicite:1]{index=1}
SASP / inflammaging readouts in vascular cells — profile cytokines and SASP factors (ELISA/RT-qPCR) in endothelial or smooth-muscle models to map short-peptide effects on aging-linked signaling. :contentReference[oaicite:2]{index=2}
Ligand-free peptide analytics — develop RP-HPLC and LC–MS/MS methods for identity, purity, recovery, and forced-degradation stability in relevant buffers and temperatures.
Cross-lineage cell models — explore AEDR responses in non-cardiac cell systems (e.g., neuron-like cells) to evaluate generalizable stress-marker modulation and viability metrics. :contentReference[oaicite:3]{index=3}

Citations (MLA)

Khavinson, V. Kh., et al. “Tetrapeptide H-Ala-Glu-Asp-Arg-OH Stimulates Expression of Cytoskeletal and Nuclear Matrix Proteins.” Bulletin of Experimental Biology and Medicine, vol. 153, no. 4, 2012, pp. 559–562. https://doi.org/10.1007/s10517-012-1766-9. :contentReference[oaicite:4]{index=4}
Menshchikova, E. B., et al. “Senescence-Associated Secretory Phenotype of Cardiovascular System: Emerging Targets.” Cells, vol. 12, no. 1, 2023, article 106. https://doi.org/10.3390/cells12010106. :contentReference[oaicite:5]{index=5}
Jevtic, M., et al. “Short Peptides Protect Fibroblast-Derived Induced Neurons from Age-Associated Changes.” International Journal of Molecular Sciences, vol. 25, no. 21, 2024, article 11363. https://doi.org/10.3390/ijms252111363. :contentReference[oaicite:6]{index=6}

Quality, Storage & Handling

Each lot is confirmed by HPLC (purity) and MS (identity); the on-vial QR links to batch COA. Independent U.S. labs verify purity, quantity, and endotoxin.

Storage (lyophilized): −20 °C long-term or 2–8 °C short-term; store desiccated and protect from light.
After reconstitution: Aliquot and store at ≤ −20 °C (or −80 °C per SOP); avoid repeated freeze–thaw cycles.
Reconstitution tips: Use sterile water or compatible buffer; gently swirl to dissolve. If sterility is required, pass through a 0.22 µm filter and aliquot promptly.

Shipping & Fulfillment

Ships with validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Disclaimer

For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines.

FAQs

What is the exact sequence and form?: H-Ala-Glu-Asp-Arg-OH (A-E-D-R), free-acid C-terminus. See the lot COA for salt form and identifiers.
Do you provide documentation?: Yes—every vial includes a QR-linked COA with HPLC/MS data, along with third-party testing for purity, quantity, and endotoxin.
How should I reconstitute?: Use sterile water or a neutral buffer. Gently invert or swirl. If your SOP requires sterility, 0.22 µm filter after dissolution and aliquot to avoid freeze–thaw cycles.
Is Cardiogen intended for human or veterinary use?: No. This product is sold strictly as RUO and must not be used in humans or animals.