NAD⁺ 1000 mg (β-Nicotinamide adenine dinucleotide; Nadide) from Peptide Tech is a premium, lyophilized research-use-only (RUO) reagent supplied at ≥99% purity (HPLC & MS verified). This oxidized dinucleotide cofactor is widely used in in-vitro redox biochemistry, enzyme kinetics, ADP-ribosylation workflows, and LC–MS metabolomics where identity, stability, and batch traceability are critical.

Specifications

Property	Details
CAS Number	53-84-9
Chemical Name	β-Nicotinamide adenine dinucleotide (oxidized form); NAD⁺; Nadide
PubChem CID	5892
Molecular Formula	C₂₁H₂₇N₇O₁₄P₂
Molecular Weight	≈ 663.43 g/mol
Physical Appearance	White to off-white lyophilized powder
Form	Oxidized dinucleotide cofactor (anhydrous)
Container	Glass vial (1000 mg net content)
Purity	≥99% verified by HPLC & MS (batch COA via on-vial QR)
Solubility	Readily soluble in sterile water and compatible neutral buffers; prepare fresh; protect from light; avoid high pH/alkaline conditions
Intended Use	Laboratory research only (RUO). Not for human or animal use.

Molecular Structure

Key Features of Peptide Tech

Same-Day Shipping (Mon–Fri, on in-stock items)
GMP-Aligned lyophilized reagents
USA Made
24/7 Support
Price Match Guarantee
Batch Tracking with QR Code Verification
3rd-Party U.S. Lab Testing — purity, quantity, endotoxin
Cold-Chain Shipping — Always (no paid upgrade)
Worldwide Shipping
All Major Payments Accepted
Text & Email Order Updates
2-Day FedEx/UPS Shipping Standard
Cheapest Overnight Rates
Largest Variety of Peptides & Bioregulators

Potential Applications in Research

Strictly in-vitro, cell-free, or cell-based workflows. No health claims or clinical/veterinary use.

Oxidoreductase enzyme kinetics (e.g., LDH, MDH, ADH) tracking NADH formation at 340 nm or via fluorescence to quantify catalytic parameters and inhibitor effects.
Sirtuin deacetylase assays that consume NAD⁺ to generate OAADPr and nicotinamide, measured by LC–MS or homogeneous fluorescence kits.
PARP/ARTD ADP-ribosylation workflows (auto- and trans-modification) with immuno/LC–MS readouts to map poly/mono-(ADP-ribose) formation in vitro.
DNA ligase (NAD⁺-dependent) assays using bacterial LigA to study nick sealing efficiency, cofactor dependence, and inhibitor screens.
Redox ratio and metabolite profiling for in-vitro metabolomics/targeted LC–MS method development (NAD⁺, NADH, NADP(H)).
Assay development & calibration (RP-HPLC/LC–MS/MS) establishing retention, ionization, and recovery of dinucleotides in buffered matrices.

Quality, Storage & Handling

Each lot is confirmed by HPLC (purity) and MS (identity); COA and batch data are accessible via QR. Independent U.S. labs verify purity, quantity, and endotoxin.

Storage (lyophilized): −20 °C long-term or 2–8 °C short-term; keep desiccated and protected from light.
After reconstitution: Use neutral, oxygen-poor buffer when possible; aliquot and store at ≤−20 °C; avoid repeated freeze–thaw.
Working solutions: Prepare fresh; minimize exposure to light/alkaline pH to reduce degradation.

Shipping & Fulfillment

Ships with validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Disclaimer

For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines.

FAQs

Is this the oxidized form?: Yes. This product is NAD⁺ (oxidized). For reduced NADH or NADP(H), see our catalog.
What purity is provided?: Each 1000 mg vial is ≥99% by HPLC with MS identity confirmation; third-party testing is performed on every lot (COA via QR).
How should I reconstitute it?: Use sterile water or a neutral, compatible buffer; gently invert to dissolve. Prepare fresh working solutions, aliquot, and avoid alkaline pH and repeated freeze–thaw.
Which in-vitro assays is NAD⁺ commonly used in?: Typical RUO uses include dehydrogenase kinetics (340 nm), sirtuin and PARP assays, NAD⁺-dependent ligase reactions, and LC–MS method development for dinucleotides.