Methylene Blue — 500 mg (Liquid) from Peptide Tech is a premium, ready-to-use research-use-only (RUO) aqueous solution of methylthioninium chloride, supplied with batch traceability and QR-linked documentation. This lab dye and redox indicator is suitable for in-vitro staining, photochemistry, electron-transfer experiments, and analytical method development where identity, stability, and light-protection matter.
Specifications
| Property | Details |
|---|---|
| Chemical Name | Methylthioninium chloride (Methylene Blue) |
| Synonyms | Basic Blue 9; Tetramethylthionine chloride; Methylene Blue |
| CAS Number | 61-73-4 |
| PubChem CID | 6099 |
| Molecular Formula | C16H18ClN3S |
| Molecular Weight | ≈ 319.85 g/mol |
| Form | Ready-to-use aqueous solution (liquid) |
| Net Content | 500 mg active dye per bottle (concentration/volume printed on label & COA) |
| Appearance | Deep blue solution; filled in light-protective (amber) container |
| Purity / ID | Identity confirmed by MS/UV-Vis; third-party verified. See lot COA via on-vial QR. |
| Sterility | 0.22 µm sterile-filtered and aseptically filled (see COA for lot-specific confirmation) |
| Solubility | Water-miscible; compatible with many neutral aqueous buffers. Protect from strong light; avoid oxidizing/strong reducing agents unless intended for the experiment. |
| Intended Use | Laboratory research only (RUO). Not for human or animal use. |
Molecular Structure
View on PubChem (CID 6099)
Key Features of Peptide Tech
- Same-Day Shipping (Mon–Fri, on in-stock items)
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- Batch Tracking with QR Code Verification
- Third-Party U.S. Lab Testing — identity, quantity, endotoxin (where applicable)
- Cold-Chain Shipping — Always (no paid upgrade)
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- 2-Day FedEx/UPS Standard with low-cost overnight
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Potential Applications in Research
Strictly in-vitro or analytical contexts. No health, diagnostic, or therapeutic claims.
- Redox & photochemistry — electron-transfer studies, photosensitization, and light-driven reactions with controlled illumination.
- UV-Vis method development — dye calibration, extinction-based quantitation, and instrument performance checks.
- Cell-free/microbiology staining — model staining and visualization protocols under controlled conditions.
- Assay QC/controls — colorimetric assay benchmarking and stability checks in buffered matrices.
- Chromatography/LC–MS sample prep — test analyte for method validation (carryover, recovery, light-stress studies).
Quality, Storage & Handling
Every lot is supported by a QR-linked COA. Independent U.S. labs verify identity and quantity, with endotoxin testing where applicable.
- Storage: 2–8 °C; protect from light; keep tightly closed. Do not autoclave the filled container.
- Handling: Use light-minimizing practices; avoid metal contamination; dye will permanently stain porous surfaces and some plastics.
- Shelf life: See lot COA and label. Use clean, dedicated tools to avoid introducing reductants/oxidants.
Shipping & Fulfillment
Ships in validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.
Compliance & RUO Disclaimer
For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines.
FAQs
- What is the concentration and volume?
- The bottle contains 500 mg of active dye in water. The exact concentration (mg/mL) and fill volume are printed on the label and listed on the COA (scan the on-vial QR).
- Is it sterile?
- Lots are 0.22 µm sterile-filtered and aseptically filled unless otherwise noted. Confirm sterility status on the COA for your lot.
- How should I store and handle it?
- Store at 2–8 °C, protected from light. Use amberware when possible, minimize light exposure during experiments, and avoid introducing strong oxidants/reductants unless required by your protocol.
- Can I use this in living systems?
- No. This product is sold strictly as RUO and is not for human or animal use.
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