Methylated B12 (Methylcobalamin) — 10 mg in 10 mL Vial, Research Grade (RUO)

Methylated B12 (Methylcobalamin, MeCbl) from Peptide Tech is supplied as a ready-to-use aqueous solution in a sealed 10 mL research vial containing 10 mg total analyte (see COA for lot-specific assay). Produced under GMP-aligned procedures with full batch traceability, this material is intended strictly for laboratory research use—ideal for in-vitro, biochemical, and analytical method development where identity, purity, light protection, and solution handling are critical.

Specifications

Property	Details
Product Name	Methylated B12 (Methylcobalamin) — 10 mg in 10 mL vial
Primary Compound	Methylcobalamin (MeCbl; methyl-vitamin B₁₂)
CAS Number	13422-55-4
PubChem CID	CID 10898559 (Methyl-vitamin B₁₂)
Molecular Formula	C₆₃H₉₁CoN₁₃O₁₄P
Molecular Weight	≈ 1344.4 g/mol
Formulation	Aqueous solution; 10 mg in 10 mL (nominal 1 mg/mL; confirm lot on COA)
Appearance	Clear to slightly red solution (cobalamin chromophore); may appear red under light
Purity (API)	≥98% typical (HPLC/MS identity confirmation per lot)
Solubility (API)	Water-soluble; protect from light; pH affects stability—see notes/citations
Container	Amber 10 mL research vial, tamper-evident closure, QR batch ID
Storage Conditions	Store refrigerated (2–8 °C), protected from light. Do not freeze unless COA indicates freeze-thaw compatibility.
Intended Use	Research Use Only (RUO) — not for human or animal use, ingestion, diagnostic, or therapeutic applications.

Molecular Structure

Key Features — Peptide Tech

Same-Day Shipping (Mon–Fri, in-stock)
GMP-Aligned solution prep & packaging
USA Made with full batch traceability
24/7 Support & Price Match Guarantee
QR COA Access — purity, ID (MS), assay, sterility/endotoxin (where applicable)
Cold-Chain Shipping — Always (no paid upgrade)
Worldwide Shipping with compliant documentation
Text & Email Updates on every order
2-Day FedEx/UPS Standard & low-cost Overnight options

Potential Applications in Research

Strictly non-clinical. Focus on in-vitro, biochemical, and analytical work; do not imply human use.

Photostability and light-protection studies of cobalamin solutions under controlled illumination; quantify degradation pathways and implement amber-vial mitigation.
pH- and solvent-stress testing with forced-degradation (acid/base, oxidative, thermal) to validate stability-indicating HPLC/LC-MS methods for MeCbl.
Spectrophotometric calibration using characteristic cobalamin absorbance bands for instrument qualification and extinction-coefficient verification.
Protein binding / carrier interaction screens (e.g., transcobalamin capture) in cell-free systems to benchmark recovery and matrix effects.
Comparative vitamer analytics (MeCbl vs. OHCbl/CNCbl) to study interconversion and relative stability under lab conditions.

Citations

Ip, K., et al. “Physicochemical Stability of Extemporaneously Prepared Methylcobalamin Injection: Light Sensitivity Considerations.” Journal of Pharmacy Practice and Research, 2019. PubMed 31085783
Lie, A. H., et al. “Sorbitol Enhances the Physicochemical Stability of B₁₂ Vitamers.” Int. J. Vitam. Nutr. Res., 2020. PDF
Al-Ogaidi, I., et al. “Photodegradation of Methylcobalamin and Its Determination in a Commercial Formulation.” Indian J. Pharm. Sci., 2018. PDF
Patel, J., et al. “Stability-Indicating RP-HPLC Method for Methylcobalamin: Degradation Kinetics & pH Effects.” J. Sep. Sci., 2022. Wiley
PubChem Compound Summary: Methyl-vitamin B₁₂ (Methylcobalamin). CID 10898559

Quality, Storage & Handling

Each lot is verified by HPLC (purity/assay) and MS (identity), with QR-linked documentation. Follow good lab practices for light-sensitive cobalamin solutions.

Storage (solution): 2–8 °C, protect from light (amber vial or foil overwrap). Do not refreeze unless COA allows; minimize headspace and repeated stopper entries.
Handling: Use low-light conditions; work behind a light shield where possible. Record extraction volumes for concentration control.
Stability notes: MeCbl stability is affected by light, heat, pH, and co-formulants; validate conditions per your stability-indicating method.

Shipping & Fulfillment

Ships in validated cold-chain packaging with amber/light-protective measures. In-stock orders placed before the daily cutoff dispatch the same day. 2-Day FedEx/UPS is standard with overnight options. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Disclaimer

For laboratory research use only. Not a drug, food, or cosmetic. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Purchaser warrants they are qualified to handle RUO materials under applicable laws and institutional policies.

FAQs

What is the concentration?: Net content is 10 mg in 10 mL (nominal 1 mg/mL). Refer to the COA for the lot-specific assay and exact concentration.

How should I protect the solution from light?: Store in the provided amber vial, keep in secondary light-protective packaging, and minimize bench-top exposure. See citations for photolability guidance.
Can I freeze the vial?: Follow the lot COA. Many aqueous MeCbl solutions are not intended for freeze–thaw; aliquot under low light if sub-volumes are needed.
Human or veterinary use?: No. This product is sold strictly as RUO and is not for human or animal use, ingestion, diagnostic, or therapeutic purposes.