Thymalin 20 mg (EW; Glu–Trp) from Peptide Tech is a premium, lyophilized research‑use‑only (RUO) dipeptide supplied at ≥99% purity (HPLC & MS verified). The sequence H‑Glu‑Trp‑OH is prepared for reproducible in‑vitro immunology/epithelium models and analytical method development—backed by batch traceability, independent third‑party testing, and validated cold‑chain logistics.

Specifications

Property	Details
Product	Thymalin — lyophilized peptide, 20 mg
Chemical Name	H‑Glu‑Trp‑OH (L‑glutamyl‑L‑tryptophan)
Synonyms	EW; Glu‑Trp; Thymogen‑like dipeptide
Sequence	Glu–Trp (E‑W)
CAS Number	38101‑59‑6 (free acid)
PubChem CID	3081371 (L‑Glu‑L‑Trp)
Molecular Formula	C₁₆H₁₉N₃O₅ (free acid)
Molecular Weight	≈ 333.34 g/mol (free acid)
Appearance	White to off‑white lyophilized powder
Container	3 mL Type I glass vial; butyl stopper; flip‑off seal
Purity / Identity	≥99% by HPLC; identity by MS (batch COA via on‑vial QR)
Counter‑ion	Typically acetate (verify on lot COA)
Solubility	Soluble in sterile water or neutral buffers; for rapid wetting at higher concentrations, pre‑wet with ≤0.1% acetic acid then bring to buffer. If sterility is required by your SOP, 0.22 µm filter after dissolution.
Storage (lyophilized)	−20 °C long‑term; store desiccated and protected from light.
After Reconstitution	Aliquot and store at ≤ −20 °C (or −80 °C per SOP); avoid repeat freeze–thaw cycles.
Intended Use	RUO only. Not for human or animal use.

Molecular Structure

Thymalin / EW (Glu–Trp) 2D structure — PubChem CID 3081371 — View on PubChem (CID 3081371)

Key Features of Peptide Tech

Same‑Day Shipping (Mon–Fri, on in‑stock items)
GMP‑Aligned lyophilization & documentation
USA‑Made peptides
24/7 Support & Price Match Guarantee
Batch QR for instant COA & chain‑of‑custody verification
3rd‑Party U.S. Lab Testing — purity, quantity, endotoxin
Cold‑Chain Shipping — Always (no paid upgrade)
Worldwide Shipping; all major payments accepted
Text & Email order updates; 2‑Day FedEx/UPS standard with low‑cost overnight
Largest Variety of peptides & bioregulators

Potential Applications in Research (RUO)

Strictly for laboratory research. No health, diagnostic, or therapeutic claims.

Immune‑cell signaling — quantify cytokine panels (e.g., IL‑6, TNF‑α, IL‑10) in macrophage or T‑cell cultures after defined EW exposures in serum‑controlled media.
Epithelial barrier & wound closure — TEER and scratch‑assay paradigms in airway/intestinal lines to evaluate barrier dynamics under standardized conditions.
Oxidative‑stress workflows — ROS quantitation, antioxidant transcripts, and mitochondrial potential in epithelial or immune models across media compositions.
Peptide transport & stability — characterize uptake and stability of short acidic dipeptides in Caco‑2/HEK systems; quantify by LC–MS(/MS).
Analytical method development — establish RP‑HPLC/LC–MS(/MS) identity, purity, recovery, and forced‑degradation stability across buffers, pH, and temperature.

Citations (selected)

Khavinson, V. Kh., et al. Short peptide bioregulators — background on thymus‑related dipeptides and epithelial/immune applications (review context).
Brandsch, M. PepT1/2 peptide transporters — methodology for di‑/tripeptide uptake studies.
Standard TEER/scratch, ROS, and LC–MS quantitation protocols (Methods in Molecular Biology/JOVE style references).

Quality, Storage & Handling

Every lot is confirmed by HPLC (purity) and MS (identity); the on‑vial QR links to the batch COA. Independent U.S. labs verify purity, quantity, and endotoxin.

Storage (lyophilized): −20 °C long‑term or 2–8 °C short‑term; keep desiccated and protected from light.
After reconstitution: Aliquot promptly; store at ≤ −20 °C (or −80 °C per SOP). Avoid multiple freeze–thaw cycles.
Reconstitution tips: Use sterile water or suitable buffer; gently swirl/invert to dissolve. If sterility is required, 0.22 µm filter and aliquot.

Shipping & Fulfillment

Ships with validated cold‑chain packaging. In‑stock orders placed before the daily cutoff ship the same day. 2‑Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted.

Compliance & RUO Disclaimer

For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Purchasers warrant appropriate qualifications and compliance with applicable regulations and institutional policies.

FAQs

Is Thymalin the same as Vilon or Thymogen?: No. Thymalin is presented here as the dipeptide Glu–Trp (EW). Vilon is Lys–Glu (KE) and Thymogen refers to Glu–Trp in some literature but is often a branded preparation; verify the exact sequence required by your assay.
Do you provide a PubChem image?: Yes—the 2D structure above is rendered from PubChem CID 3081371 for L‑Glu‑L‑Trp (EW).
What purity do you provide?: Each 20 mg vial is ≥99% by HPLC with MS identity confirmation; third‑party testing is performed on every lot (COA via on‑vial QR).
How should I reconstitute it?: Dissolve in sterile water or a compatible buffer; gently invert until fully dissolved. If your SOP requires sterility, 0.22 µm filter and aliquot to avoid repeat freeze–thaw.